Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl)
- PMID: 12789442
- DOI: 10.1007/s00299-002-0544-y
Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl)
Abstract
Regeneration via shoot organogenesis and somatic embryogenesis was observed from thidiazuron (TDZ)-treated leaf and petiole explants of greenhouse- and in vitro-grown African violet plants. The response of cultures to other growth regulators over a range of 0.5 microM to 10 microM was 50% less than that observed with TDZ. A comparative study among several cultivars of African violet indicated that "Benjamin" and "William" had the highest regeneration potential. In "Benjamin", higher frequencies of shoot organogenesis (twofold) and somatic embryogenesis (a 50% increase) were observed from in vitro- and greenhouse-grown plants, respectively. At concentrations lower than 2.5 microM, TDZ induced shoot organogenesis, whereas at higher doses (5-10 microM) somatic embryos were formed. These findings provide the first report of simultaneous shoot organogenesis and somatic embryogenesis of African violet explants in response to TDZ.
Similar articles
-
Thidiazuron-induced abnormalities in plant tissue cultures.Plant Cell Rep. 2018 Nov;37(11):1451-1470. doi: 10.1007/s00299-018-2326-1. Epub 2018 Jul 26. Plant Cell Rep. 2018. PMID: 30051285 Review.
-
Micropropagation of African violet (Saintpaulia ionantha Wendl.).Methods Mol Biol. 2013;11013:279-89. doi: 10.1007/978-1-62703-074-8_22. Methods Mol Biol. 2013. PMID: 23179707
-
Thidiazuron-induced morphogenetic response in petiole cultures of Pelargonium x hortorum and Pelargonium x domesticum and its histological analysis.Plant Cell Rep. 2004 Oct;23(4):211-7. doi: 10.1007/s00299-004-0844-5. Epub 2004 Aug 5. Plant Cell Rep. 2004. PMID: 15300403
-
Artemisinin production by shoot regeneration of Artemisia annua L. using thidiazuron.Z Naturforsch C J Biosci. 2008 Jan-Feb;63(1-2):96-100. doi: 10.1515/znc-2008-1-218. Z Naturforsch C J Biosci. 2008. PMID: 18386496
-
De novo shoot organogenesis during plant regeneration.J Exp Bot. 2020 Jan 1;71(1):63-72. doi: 10.1093/jxb/erz395. J Exp Bot. 2020. PMID: 31504722 Review.
Cited by
-
Thidiazuron-induced regeneration of Echinacea purpurea L.: micropropagation in solid and liquid culture systems.Plant Cell Rep. 2007 Jan;26(1):13-9. doi: 10.1007/s00299-006-0209-3. Epub 2006 Aug 2. Plant Cell Rep. 2007. PMID: 16897009
-
TDZ-induced direct shoot organogenesis and somatic embryogenesis on cotyledonary node explants of lentil (Lens culinaris Medik.).Physiol Mol Biol Plants. 2008 Oct;14(4):347-53. doi: 10.1007/s12298-008-0033-z. Epub 2009 Feb 26. Physiol Mol Biol Plants. 2008. PMID: 23572901 Free PMC article.
-
Thidiazuron-induced somatic embryogenesis and changes of antioxidant properties in tissue cultures of half-high blueberry plants.Sci Rep. 2018 Nov 19;8(1):16978. doi: 10.1038/s41598-018-35233-6. Sci Rep. 2018. PMID: 30451961 Free PMC article.
-
Thidiazuron-induced abnormalities in plant tissue cultures.Plant Cell Rep. 2018 Nov;37(11):1451-1470. doi: 10.1007/s00299-018-2326-1. Epub 2018 Jul 26. Plant Cell Rep. 2018. PMID: 30051285 Review.
-
Mapping quantitative trait loci for tissue culture response in VCS3M-DH population of Brassica rapa.Plant Cell Rep. 2013 Aug;32(8):1251-61. doi: 10.1007/s00299-013-1433-2. Epub 2013 Apr 6. Plant Cell Rep. 2013. PMID: 23563522
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
